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- References [29]
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- Immunohistochemistry [1]
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- Product number
- 20225-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#20225-1-AP, RRID:AB_10646481
- Product name
- ARF6-Specific antibody
- Antibody type
- Polyclonal
- Description
- ARF6-Specific antibody (Cat. #20225-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Lysophosphatidic acid-induced Arf6-driven macropinocytosis of CD147(+) extracellular vesicles promotes sorafenib resistance of hepatocellular carcinoma.
A tandem recruitment site in the pseudokinase scaffold PEAK3 is subject to phosphorylation-dependent regulation and cancer-associated mutations.
ToF-SIMS revealing sphingolipids composition in extracellular vesicles and paternal β-cells after persistent hyperglycemia.
Magnetic-Assisted Aptamer Selection and Isolation of Migrasomes for Application in Lung Adenocarcinoma Proteomic Analysis.
Acute aerobic exercise alters serum protein distribution in colorectal cancer patients.
ARF6 Promotes AML Progression via Activation of PI3K/AKT/mTOR Signaling.
miR-28-3p suppresses gastric cancer growth and EMT-driven metastasis by targeting the ARF6/Hedgehog axis.
Aberrant expression of GTPase-activating protein ARAP1 triggers circular dorsal ruffles associated with malignancy in hepatocellular carcinoma Hep3B cells.
Emerging of Ultrafine Membraneless Organelles as the Missing Piece of Nanostress: Mechanism of Biogenesis and Implications at Multilevels.
Liver-breast communication of adipocyte-oriented exosomes drives primary mammary cancer progression.
G3BP1 maintains endothelial barrier integrity through dual mechanisms: direct stabilization of junction protein mRNAs and suppression of the inflammatory MYD88-ARNO-ARF6 pathway.
RAB22A sorts epithelial growth factor receptor (EGFR) from early endosomes to recycling endosomes for microvesicles release.
CD24 promotes metastasis and chemoresistance by directly targeting Arf6-ERK pathway in esophageal squamous cell carcinoma.
Uncovering the Importance of Ligand Mobility on Cellular Uptake of Nanoparticles: Insights from Experimental, Computational, and Theoretical Investigations.
ARF6, a component of intercellular bridges, is essential for spermatogenesis in mice.
Platelet-derived extracellular vesicles aggravate septic acute kidney injury via delivering ARF6.
Extracellular HSPA5 is autocrinally involved in the regulation of neuronal process elongation.
Distribution of Signal Peptides in Microvesicles from Activated Macrophage Cells.
ARF6 plays a general role in targeting palmitoylated proteins from the Golgi to the plasma membrane.
Targeting EGFR-dependent tumors by disrupting an ARF6-mediated sorting system.
Recruitment of DNA to tumor-derived microvesicles.
Astragalus membranaceus (Huangqi) and Rhizoma curcumae (Ezhu) decoction suppresses colorectal cancer via downregulation of Wnt5/β-Catenin signal.
XIST sponges miR-320d to promote chordoma progression by regulating ARF6.
Subcellular localization of glypican-5 is associated with dynamic motility of the human mesenchymal stem cell line U3DT.
An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.
Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity.
ADP-ribosylation factor 1 expression regulates epithelial-mesenchymal transition and predicts poor clinical outcome in triple-negative breast cancer.
TNF stimulates nuclear export and secretion of IL-15 by acting on CRM1 and ARF6.
BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein modulates ATP-binding cassette transporter A-1 trafficking and function.
Qian L, Fu Z, Chen P, Guo Y, Li Y, Wang Y, Wang B, Zhang Q, Guo Q, Wu L, Kucharzewska P, Chen Z, Piao Y, Zhang S
International journal of biological sciences 2026;22(1):220-238
International journal of biological sciences 2026;22(1):220-238
A tandem recruitment site in the pseudokinase scaffold PEAK3 is subject to phosphorylation-dependent regulation and cancer-associated mutations.
Zhao T, Hou J, Hu C, Cotton TR, Daly RJ
The Journal of biological chemistry 2026 Mar 10;302(5):111365
The Journal of biological chemistry 2026 Mar 10;302(5):111365
ToF-SIMS revealing sphingolipids composition in extracellular vesicles and paternal β-cells after persistent hyperglycemia.
Skalska ME, Durak-Kozica M, Stępień EŁ
Talanta 2026 Jan 1;297(Pt A):128582
Talanta 2026 Jan 1;297(Pt A):128582
Magnetic-Assisted Aptamer Selection and Isolation of Migrasomes for Application in Lung Adenocarcinoma Proteomic Analysis.
Xu Y, Jiao L, Yi Q, Chen Y, Cai B, Zhang J, Huang Z, Luo Y, Si Y, Wu Y, Ying B, Chen J, Zhou J
Journal of extracellular vesicles 2026 Apr;15(4):e70283
Journal of extracellular vesicles 2026 Apr;15(4):e70283
Acute aerobic exercise alters serum protein distribution in colorectal cancer patients.
Jin S, Han S, Wang N, Yang M, Chen C
Frontiers in oncology 2025;15:1586344
Frontiers in oncology 2025;15:1586344
ARF6 Promotes AML Progression via Activation of PI3K/AKT/mTOR Signaling.
Xu H, Chen D, Lu J, Zhong L, Wang L, Ge J
Cancer medicine 2025 May;14(9):e70872
Cancer medicine 2025 May;14(9):e70872
miR-28-3p suppresses gastric cancer growth and EMT-driven metastasis by targeting the ARF6/Hedgehog axis.
Ji H, Liu S, Yang L, Wu Y, Zhang H, Liu X, Li L, Li L
Molecular and cellular probes 2025 Feb;79:102010
Molecular and cellular probes 2025 Feb;79:102010
Aberrant expression of GTPase-activating protein ARAP1 triggers circular dorsal ruffles associated with malignancy in hepatocellular carcinoma Hep3B cells.
Sun X, Li Y, He Y, Cheng L, Wang L, Wei J, Chen J, Du L, Shen Z, Xie Y, Midgley AC, Jiang W, Yoshida S
Cell communication and signaling : CCS 2025 Feb 11;23(1):75
Cell communication and signaling : CCS 2025 Feb 11;23(1):75
Emerging of Ultrafine Membraneless Organelles as the Missing Piece of Nanostress: Mechanism of Biogenesis and Implications at Multilevels.
Liu J, Zheng L, Li X, Tang W, Guo M, Wang Y, Tan X, Chang J, Zhao H, Zhu D, Ma YQ, Huo D
ACS nano 2025 Feb 11;19(5):5659-5679
ACS nano 2025 Feb 11;19(5):5659-5679
Liver-breast communication of adipocyte-oriented exosomes drives primary mammary cancer progression.
Li C, Lu Y, Li Y, Liu T, Deng H, Gao M, Zhou B, Liu J, Cai J, Huang D, Yang L, Jin J, Kuang D, Su S
Cell metabolism 2025 Dec 2;37(12):2402-2422.e18
Cell metabolism 2025 Dec 2;37(12):2402-2422.e18
G3BP1 maintains endothelial barrier integrity through dual mechanisms: direct stabilization of junction protein mRNAs and suppression of the inflammatory MYD88-ARNO-ARF6 pathway.
Sun W, Wu H, He Y, Chen H, Meng Y, Tang G, Zhu J, Wen Z, Zhang H, Wu R, Wu G, Zhang C, Chu M, Wen B
Angiogenesis 2025 Aug 25;28(4):46
Angiogenesis 2025 Aug 25;28(4):46
RAB22A sorts epithelial growth factor receptor (EGFR) from early endosomes to recycling endosomes for microvesicles release.
Lin Y, Wei D, He X, Huo L, Wang J, Zhang X, Wu Y, Zhang R, Gao Y, Kang T
Journal of extracellular vesicles 2024 Jul;13(7):e12494
Journal of extracellular vesicles 2024 Jul;13(7):e12494
CD24 promotes metastasis and chemoresistance by directly targeting Arf6-ERK pathway in esophageal squamous cell carcinoma.
Hong P, Xu T, Xu J, Chen W, Hu H, Chen J, Li L, Zheng C, Li B, Liu J, Dai W, Li E, Zhang F, Xu W
Cancer letters 2024 Jul 10;594:216994
Cancer letters 2024 Jul 10;594:216994
Uncovering the Importance of Ligand Mobility on Cellular Uptake of Nanoparticles: Insights from Experimental, Computational, and Theoretical Investigations.
Chen YQ, Xue MD, Li JL, Huo D, Ding HM, Ma Y
ACS nano 2024 Feb 27;18(8):6463-6476
ACS nano 2024 Feb 27;18(8):6463-6476
ARF6, a component of intercellular bridges, is essential for spermatogenesis in mice.
Wong HN, Chen T, Wang PJ, Holzman LB
Developmental biology 2024 Apr;508:46-63
Developmental biology 2024 Apr;508:46-63
Platelet-derived extracellular vesicles aggravate septic acute kidney injury via delivering ARF6.
Lu X, Jiang G, Gao Y, Chen Q, Sun S, Mao W, Zhang N, Zhu Z, Wang D, Zhang G, Chen M, Zhang L, Chen S
International journal of biological sciences 2023;19(16):5055-5073
International journal of biological sciences 2023;19(16):5055-5073
Extracellular HSPA5 is autocrinally involved in the regulation of neuronal process elongation.
Fukawa M, Shirai R, Torii T, Nakata K, Fukatsu S, Sato T, Homma K, Miyamoto Y, Yamauchi J
Biochemical and biophysical research communications 2023 Jul 5;664:50-58
Biochemical and biophysical research communications 2023 Jul 5;664:50-58
Distribution of Signal Peptides in Microvesicles from Activated Macrophage Cells.
Ono K, Sato J, Suzuki H, Sawada M
International journal of molecular sciences 2023 Jul 28;24(15)
International journal of molecular sciences 2023 Jul 28;24(15)
ARF6 plays a general role in targeting palmitoylated proteins from the Golgi to the plasma membrane.
Wang J, Zheng LF, Ren S, Li DL, Chen C, Sun HH, Liu LY, Guo H, Zhao TJ
Journal of cell science 2023 Aug 1;136(15)
Journal of cell science 2023 Aug 1;136(15)
Targeting EGFR-dependent tumors by disrupting an ARF6-mediated sorting system.
Guo H, Wang J, Ren S, Zheng LF, Zhuang YX, Li DL, Sun HH, Liu LY, Xie C, Wu YY, Wang HR, Deng X, Li P, Zhao TJ
Nature communications 2022 Oct 12;13(1):6004
Nature communications 2022 Oct 12;13(1):6004
Recruitment of DNA to tumor-derived microvesicles.
Clancy JW, Sheehan CS, Boomgarden AC, D'Souza-Schorey C
Cell reports 2022 Mar 1;38(9):110443
Cell reports 2022 Mar 1;38(9):110443
Astragalus membranaceus (Huangqi) and Rhizoma curcumae (Ezhu) decoction suppresses colorectal cancer via downregulation of Wnt5/β-Catenin signal.
Bian Y, Wang G, Zhou J, Yin G, Liu T, Liang L, Yang X, Zhang W, Ni K, Tang D, Yu Y
Chinese medicine 2022 Jan 6;17(1):11
Chinese medicine 2022 Jan 6;17(1):11
XIST sponges miR-320d to promote chordoma progression by regulating ARF6.
Wang Y, Tang Z, Guo W
Journal of bone oncology 2022 Aug;35:100447
Journal of bone oncology 2022 Aug;35:100447
Subcellular localization of glypican-5 is associated with dynamic motility of the human mesenchymal stem cell line U3DT.
Takeuchi M, Takeuchi K, Takai T, Yamaguchi R, Furukawa T, Akagi KI, Takeuchi JK
PloS one 2021;16(2):e0226538
PloS one 2021;16(2):e0226538
An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.
Clancy JW, Zhang Y, Sheehan C, D'Souza-Schorey C
Nature cell biology 2019 Jul;21(7):856-866
Nature cell biology 2019 Jul;21(7):856-866
Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity.
Candiello E, Mishra R, Schmidt B, Jahn O, Schu P
Scientific reports 2017 Nov 17;7(1):15781
Scientific reports 2017 Nov 17;7(1):15781
ADP-ribosylation factor 1 expression regulates epithelial-mesenchymal transition and predicts poor clinical outcome in triple-negative breast cancer.
Schlienger S, Campbell S, Pasquin S, Gaboury L, Claing A
Oncotarget 2016 Mar 29;7(13):15811-27
Oncotarget 2016 Mar 29;7(13):15811-27
TNF stimulates nuclear export and secretion of IL-15 by acting on CRM1 and ARF6.
Ouyang S, Hsuchou H, Kastin AJ, Pan W
PloS one 2013;8(8):e69356
PloS one 2013;8(8):e69356
BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein modulates ATP-binding cassette transporter A-1 trafficking and function.
Lin S, Zhou C, Neufeld E, Wang YH, Xu SW, Lu L, Wang Y, Liu ZP, Li D, Li C, Chen S, Le K, Huang H, Liu P, Moss J, Vaughan M, Shen X
Arteriosclerosis, thrombosis, and vascular biology 2013 Feb;33(2):e31-8
Arteriosclerosis, thrombosis, and vascular biology 2013 Feb;33(2):e31-8
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human liver tissue were subjected to SDS PAGE followed by western blot with 20225-1-AP(ARF6-Specific antibody) at dilution of 1:300
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The ARF6-Specific antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human ARF6-Specific. This antibody recognizes human,mouse,rat antigen. The ARF6-Specific antibody has been validated for the following applications: ELISA, WB, IHC analysis.
