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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP8146a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP8146A, RRID:AB_2299491
- Product name
- PFKFB2 Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Label-free quantitative proteomic analysis of right ventricular remodeling in infant Tetralogy of Fallot patients.
Expression, regulation and function of phosphofructo-kinase/fructose-biphosphatases (PFKFBs) in glucocorticoid-induced apoptosis of acute lymphoblastic leukemia cells.
Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases.
Xia Y, Hong H, Ye L, Wang Y, Chen H, Liu J
Journal of proteomics 2013 Jun 12;84:78-91
Journal of proteomics 2013 Jun 12;84:78-91
Expression, regulation and function of phosphofructo-kinase/fructose-biphosphatases (PFKFBs) in glucocorticoid-induced apoptosis of acute lymphoblastic leukemia cells.
Carlet M, Janjetovic K, Rainer J, Schmidt S, Panzer-Grümayer R, Mann G, Prelog M, Meister B, Ploner C, Kofler R
BMC cancer 2010 Nov 23;10:638
BMC cancer 2010 Nov 23;10:638
Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases.
Yalcin A, Clem BF, Simmons A, Lane A, Nelson K, Clem AL, Brock E, Siow D, Wattenberg B, Telang S, Chesney J
The Journal of biological chemistry 2009 Sep 4;284(36):24223-32
The Journal of biological chemistry 2009 Sep 4;284(36):24223-32
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of anti-PFKFB2 Pab (Cat. #AP8146a) in mouse kidney tissue lysate (35ug/lane). PFKFB2(arrow) was detected using the purified Pab
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
