PA5-97204

antibody from Invitrogen Antibodies

Targeting: PFKFB2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97204

Provider

Invitrogen Antibodies

Product name

PFKFB2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: RDKPTNNFPK NQTPVRMRRN SFTPLSSSNT IRRPRNYSVG SRPLKPLSPL RAQDMQEGAD; Positive Samples: A-549, Mouse liver, Mouse brain, Mouse skeletal muscle, Rat liver

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.68 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PFKFB2 in extracts of various cell lines. Samples were incubated with PFKFB2 Polyclonal antibody (Product # PA5-97204) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PFKFB2 in C6 cells. Samples were incubated with PFKFB2 Polyclonal antibody (Product # PA5-97204) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PFKFB2 in L929 cells. Samples were incubated with PFKFB2 Polyclonal antibody (Product # PA5-97204) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.