sc-735

antibody from Santa Cruz Biotechnology

Targeting: TAF1 BA2R, CCG1, CCGS, DYT3, DYT3/TAF1, KAT4, NSCL2, TAF2A, TAFII250

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-735

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-735, RRID:AB_671202

Product name

Anti-TAF1

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

References

Submitted references

The human SPT20-containing SAGA complex plays a direct role in the regulation of endoplasmic reticulum stress-induced genes.

Nagy Z, Riss A, Romier C, le Guezennec X, Dongre AR, Orpinell M, Han J, Stunnenberg H, Tora L
Molecular and cellular biology 2009 Mar;29(6):1649-60

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STAGA recruits Mediator to the MYC oncoprotein to stimulate transcription and cell proliferation.

Liu X, Vorontchikhina M, Wang YL, Faiola F, Martinez E
Molecular and cellular biology 2008 Jan;28(1):108-21

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A comprehensive ChIP-chip analysis of E2F1, E2F4, and E2F6 in normal and tumor cells reveals interchangeable roles of E2F family members.

Xu X, Bieda M, Jin VX, Rabinovich A, Oberley MJ, Green R, Farnham PJ
Genome research 2007 Nov;17(11):1550-61

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Identification of novel functional TBP-binding sites and general factor repertoires.

Denissov S, van Driel M, Voit R, Hekkelman M, Hulsen T, Hernandez N, Grummt I, Wehrens R, Stunnenberg H
The EMBO journal 2007 Feb 21;26(4):944-54

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A high-resolution map of active promoters in the human genome

Tae Hoon Kim, Leah O. Barrera, Ming Zheng, Chunxu Qu, Michael A. Singer, Todd A. Richmond, Yingnian Wu, Roland D. Green, Bing Ren
Nature 2005 Jun;436(7052):876-880

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Direct isolation and identification of promoters in the human genome.

Kim TH, Barrera LO, Qu C, Van Calcar S, Trinklein ND, Cooper SJ, Luna RM, Glass CK, Rosenfeld MG, Myers RM, Ren B
Genome research 2005 Jun;15(6):830-9

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HIV-1 Tat stimulates transcription complex assembly through recruitment of TBP in the absence of TAFs.

Raha T, Cheng SW, Green MR
PLoS biology 2005 Feb;3(2):e44

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Ras/mitogen-activated protein kinase signaling activates Ets-1 and Ets-2 by CBP/p300 recruitment.

Foulds CE, Nelson ML, Blaszczak AG, Graves BJ
Molecular and cellular biology 2004 Dec;24(24):10954-64

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A global transcriptional regulatory role for c-Myc in Burkitt's lymphoma cells.

Li Z, Van Calcar S, Qu C, Cavenee WK, Zhang MQ, Ren B
Proceedings of the National Academy of Sciences of the United States of America 2003 Jul 8;100(14):8164-9

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Evidence that TAF-TATA box-binding protein interactions are required for activated transcription in mammalian cells.

Martel LS, Brown HJ, Berk AJ
Molecular and cellular biology 2002 Apr;22(8):2788-98

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 213

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.