GTX48659
antibody from GeneTex
Targeting: TAF1
BA2R, CCG1, CCGS, DYT3, DYT3/TAF1, KAT4, NSCL2, TAF2A, TAFII250
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [2]
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Validation data
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- Product number
- GTX48659 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48659, RRID:AB_11171844
- Product name
- TAF1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's affinity purified anti-TAF1 to detect TAF1 in HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3 and 1:1,000 (Lane 4). The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1. Personal Communication, Anne Gegonne, CCR-NCI, Bethesda, MD.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody (GTX48659) was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). ChIP was performed on fos promoters using 5 ?g of TAF1 antibody(GTX48659) and an RNA PolII antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody (GTX48659) was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). ChIP was performed on fos promoters using 5 ?g of TAF1 antibody(GTX48659) and an RNA PolII antibody.