600-401-995

antibody from Invitrogen Antibodies

Targeting: TAF1 BA2R, CCG1, CCGS, DYT3, DYT3/TAF1, KAT4, NSCL2, TAF2A, TAFII250

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-995

Provider

Invitrogen Antibodies

Product name

TAF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1.1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot using Rocklands affinity purified anti-TAF1 to detect TAF1 in HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3 and 1:1,000 (Lane 4). The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1. Personal Communication, Anne Gegonne, CCR-NCI, Bethesda, MD.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of rabbit anti-TAF1 antibody. Tissue: prostate. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-TAF1 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: TAF-1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). CHIP was performed on fos promoters using 5 µg of TAF1 antibody from Rockland (P/n 600-401-995) and an RNA PolII antibody. Image with data provided courtesy of Shiraz Mujtaba, Ph.D., Dept. of Structural & Chemical Biology, Mount Sinai School of Medicine.