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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- GTX79225 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX79225, RRID:AB_11177294
- Product name
- Synaptophysin antibody [SP11] (ready-to-use)
- Antibody type
- Monoclonal
- Host
- Rabbit
- Vial size
- 6 ml
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- Experimental details
- Flow cytometry analysis of TCR V alpha 2 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with TCR V alpha 2 antibody [F1] (FITC) at a dilution of 1:10 for 60 min at room temperature and re-suspended in PBS for FACS analysis.
