H00006843-M02

antibody from Abnova Corporation

Targeting: VAMP1 SYB1, VAMP-1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006843-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006843-M02, RRID:AB_566266

Product name

VAMP1 monoclonal antibody (M02), clone 5A4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant VAMP1.

Antigen sequence

MTSNRRLQQTQAQVEEVVDIIRVNVDKVLERDQKL
SELDDRADALQAGASQFESSAAKLKRKYWWKNCK


Isotype

IgG

Antibody clone number

5A4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

VAMP1 monoclonal antibody (M02), clone 5A4 Western Blot analysis of VAMP1 expression in HepG2 ( Cat # L019V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged VAMP1 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to VAMP1 on HepG2 cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to VAMP1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 1.5 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol