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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
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- Product number
- 702787 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VAMP1 Recombinant Rabbit Monoclonal Antibody (24H9L10)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 24H9L10
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, PC12 cells were fixed and permeabilized for detection of endogenous VAMP1 using Anti- VAMP1 Recombinant Rabbit Monoclonal Antibody (Product # 702787, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of VAMP1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). anel c) is a composite image of Panels a and b clearly demonstrating localisation of VAMP-1 in cytoplamic vesicles. The images were captured at 100X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, PC12 cells were fixed and permeabilized for detection of endogenous VAMP1 using Anti-VAMP1 Recombinant Rabbit Monoclonal Antibody (Product # 702787, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) is stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows representative undifferentiated cells that were stained for detection and localization of VAMP1 protein (green) with no signal. Panel e-h) clearly demonstrate enhanced signal of VMAP1 in cell body and neuronal processes of NGF (200 ng/mL 5days) induced differentiated PC12 cells. The images were captured at 60X magnification.
