A300-948A

antibody from Invitrogen Antibodies

Targeting: DTL CDT2, DCAF2, L2DTL, RAMP

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-948A

Provider

Invitrogen Antibodies

Product name

DTL/CDT2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DTL/CDT2 by western blot. Samples: Whole cell lysate (15 µg) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DTL/CDT2 antibody (Product # A300-948A; lot A300-948A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DTL/CDT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DTL/CDT2 antibody A300-948A (lot A300-948A-2) used for IP at 6 µg per reaction. DTL/CDT2 was also immunoprecipitated by rabbit anti-DTL/CDT2 antibody A300-947A. For blotting immunoprecipitated DTL/CDT2, A300-948A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DTL/CDT2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DTL/CDT2 antibody A300-948A (lot A300-948A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse DTL/CDT2 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma.Antibody: Affinity purified rabbit anti-DTL/CDT2 (Product # A300-948A; lot 3) used at a dilution of 1:200 (1 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DTL/CDT2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma.Antibody: Affinity purified rabbit anti-DTL/CDT2 (Product # A300-948A lot 3) used at a dilution of 1:1000 (0.2 µg/mL).Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse DTL/CDT2 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma.Antibody: Affinity purified rabbit anti-DTL/CDT2 (Cat. No. A300-948A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DTL/CDT2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma.Antibody: Affinity purified rabbit anti-DTL/CDT2 (Cat. No. A300-948A Lot2) used at a dilution of 1:200 (1µg/ml).Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DTL/CDT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DTL/CDT2 antibody (Product # A300-948A; lot A300-948A-3) used for IP at 6 µg per reaction. DTL/CDT2 was also immunoprecipitated by a previous lot of this antibody (lot A300-948A-2). For blotting immunoprecipitated DTL/CDT2 (Product # A300-948A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.