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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- PA5-21571 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-DC8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, C8D30, Rat2. Predicted reactivity: Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DC8 using 30 µg C8D30 whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a DC8 polyclonal antibody (Product # PA5-21571) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DC8 using 30 µg Rat2 whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a DC8 polyclonal antibody (Product # PA5-21571) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DC8 using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a DC8 polyclonal antibody (Product # PA5-21571) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DC8 in paraformaldehyde-fixed HeLa cells using a DC8 polyclonal antibody (Product # PA5-21571) at a 1:200 dilution.
