F47894

antibody from NSJ Bioreagents

Targeting: PROX1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

F47894

Provider

NSJ Bioreagents

Product name

PROX1 Antibody / Prospero homeobox 1

Antibody type

Polyclonal

Description

This highly specific PROX1 antibody is suitable for use in Western blot/Immunofluorescence/ELISA applications with human and rat samples.

Reactivity

Human, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the PROX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of lysate from (1) human A2058 and (2) rat PC-12 cell line using PROX1 antibody at 1:1000. Predicted molecular weight is 83 kDa, observed at 80-110 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

PROX1 antibody western blot analysis in A2058 lysate

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of PROX1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Fluorescent confocal image of A2058 cell stained with PROX1 antibody at 1:25. PROX1 immunoreactivity is localized to the nucleus.