F47897

antibody from NSJ Bioreagents

Targeting: PROX1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F47897

Provider

NSJ Bioreagents

Product name

PROX1 Antibody / Prospero homeobox 1

Antibody type

Polyclonal

Description

This highly specific PROX1 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/ELISA applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the PROX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of PROX1 antibody at 1:2000 dilution. Lane 1: HepG2 lysate; 2: mouse liver lysate; 3: SH-SY5Y lysate; Predicted molecular weight is 83 kDa, observed at 80-110 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of PROX1 antibody at 1:2000 dilution. Lane 1: SW620 lysate; 2: SH-SY5Y lysate

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

PROX1 antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

PROX1 antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.