PA5-85552

antibody from Invitrogen Antibodies

Targeting: PROX1

Antibody data

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Validation data

Product number: PA5-85552 - Provider product page
Provider: Invitrogen Antibodies
Product name: PROX1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Keep as concentrated solution. Predicted reactivity: Mouse (97%), Rat (97%), Pig (97%), Chicken (89%), Rhesus Monkey (100%), Bovine (97%). Positive Control: HepG2 nuclear extract, mouse hippocampus, mouse eye, fetal mouse forebrain. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

PROX1 protein structure - PA5-85552 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PROX1 in mouse tissue extract using PROX1 polyclonal antibody (Product # PA5-85552) using 50 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PROX1 in HepG2 whole cell extract and conditioned medium using PROX1 polyclonal antibody (Product # PA5-85552) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PROX1 in A) 50 µg fetal mouse forebrain lysate using PROX1 polyclonal antibody (Product # PA5-85552) using 50 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PROX1 in mouse tissue extract using PROX1 polyclonal antibody (Product # PA5-85552) using 50 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using PROX1 Polyclonal Antibody (Product # PA5-85552). Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PROX1 Polyclonal Antibody (Product # PA5-85552) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using PROX1 Polyclonal Antibody (Product # PA5-85552). HepG2 whole cell and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PROX1 Polyclonal Antibody (Product # PA5-85552) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using PROX1 Polyclonal Antibody (Product # PA5-85552). Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PROX1 Polyclonal Antibody (Product # PA5-85552) diluted at 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: PROX1 Polyclonal Antibody detects PROX1 protein by western blot analysis. A. 50 µg fetal mouse forebrain lysate/extract.7.5 % SDS-PAGE. PROX1 Polyclonal Antibody (Product # PA5-85552) dilution: 1:1,000.