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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Flow cytometry [2]
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- Product number
- RQ6790 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Prospero homeobox protein 1 Antibody / PROX1
- Antibody type
- Polyclonal
- Description
- This highly specific Prospero homeobox protein 1 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Prospero homeobox protein 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HepG2, 2) human 293T, 3) human K562, 4) human PC-3, 5) rat brain and 6) mouse brain tissue lysate with Prospero homeobox protein 1 antibody. Predicted molecular weight is 83 kDa, observed at 80-110 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HepG2, 2) human 293T, 3) human K562, 4) human PC-3 and 5) rat brain tissue lysate with Prospero homeobox protein 1 antibody. Predicted molecular weight is 83 kDa, observed at 80-110 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HepG2, 2) human HUH7, 3) human SH-SY5Y and 4) rat RH35 cell lysate with Prospero homeobox protein 1 antibody. Predicted molecular weight is 83 kDa, observed at 80-110 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human SiHa cells with Prospero homeobox protein 1 antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Enhanced validation
- Submitted by
- NSJ Bioreagents (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation of Prospero homeobox protein 1 protein from 500ug of human SH-SY5Y whole cell lysate with 2ug of Prospero homeobox protein 1 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human K562 cells with Prospero homeobox protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Prospero homeobox protein 1 antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with Prospero homeobox protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Prospero homeobox protein 1 antibody.
