MA5-31871

antibody from Invitrogen Antibodies

Targeting: PTPN14 PEZ

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31871

Provider

Invitrogen Antibodies

Product name

PTPN14 Monoclonal Antibody (2B3H7)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-31871 has been tested in indirect ELISA.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2B3H7

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Physical Interaction between HPV16E7 and the Actin-Binding Protein Gelsolin Regulates Epithelial-Mesenchymal Transition via HIPPO-YAP Axis.

Matarrese P, Vona R, Ascione B, Paggi MG, Mileo AM
Cancers 2021 Jan 19;13(2)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PTPN14 in human PTPN14 recombinant protein. Sample was incubated with PTPN14 monoclonal antibody (Product # MA5-31871) using a dilution of 1:500-1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PTPN14 in HEK293 (1) and PTPN14 cell lysate. Samples were incubated with PTPN14 monoclonal antibody (Product # MA5-31871) using a dilution of 1:500-1:2000.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of PTPN14 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with PTPN14 monoclonal antibody (Product # MA5-31871) using a dilution of 1:10,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PTPN14 in Hela cells (green). Sample was incubated with PTPN14 monoclonal antibody (Product # MA5-31871) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of PTPN14 in HeLa cells (green). Sample was incubated with PTPN14 monoclonal antibody (Product # MA5-31871) using a dilution of 1:200-1:400 followed by negative control (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Specific relevance of E7-GSN interaction. ( A ) Representative western blot of endogenous gelsolin (GSN) knockdown showing the relative abundance of GSN in pAmCyan trasfected cells (Mock), in transfected cells treated with specific siRNAs for GSN silencing (siRNAGSN) or treated with scrambled siRNAs as a negative control (siRNAcontrol) (left). Flow cytometry evaluation of the intracellular amount of F-actin restricted to pAmCyan-positive cells transfected with siRNAGSN or scrambled siRNA as control. Histograms obtained in a representative experiment are shown. Bar graph (right) shows the mean +- SD of the median fluorescence intensity obtained in four different experiments. ** p < 0.01 and *** p < 0.001 vs. Mock transfected cells. ( B - D ) Quantitative evaluation of PTPN14/E7 molecular association by FRET technique. ( B ) Flow cytometry analysis was restricted to pAmCyan-positive cells (reported as percentage). Bar graph in ( C ) shows FRET efficiency calculated according to the Riemann's algorithm. Data are reported as mean +- SD from three independent acquisitions. ( D ) Cytofluorimetric histograms obtained in one experiment representative of three. Numbers in the first and second columns indicate the median fluorescence intensity of the respective proteins, the percentage in the third column represent FL3 positive events. Uncropped western blot images for Figure 4 A are available in Supplementary Figure S8 .