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- Antigen structure
- References [15]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- AF3059 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse S100A8 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) S100A9, rmS100A10, and recombinant human S100B is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P27005- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references C-terminal truncation of IFN-γ inhibits proinflammatory macrophage responses and is deficient in autoimmune disease.
HIF-1α activation in myeloid cells accelerates dextran sodium sulfate-induced colitis progression in mice.
Beneficial Effects of Sodium Phenylbutyrate Administration during Infection with Salmonella enterica Serovar Typhimurium.
Exploring experimental cerebral malaria pathogenesis through the characterisation of host-derived plasma microparticle protein content.
Loss of JUNB/AP-1 promotes invasive prostate cancer.
S100A9 knockout decreases the memory impairment and neuropathology in crossbreed mice of Tg2576 and S100A9 knockout mice model.
Preferential lymphatic growth in bronchus-associated lymphoid tissue in sustained lung inflammation.
The junctional epithelium originates from the odontogenic epithelium of an erupted tooth.
Neutrophil dependence of vascular remodeling after Mycoplasma infection of mouse airways.
Vaginal epithelial cell-derived S100 alarmins induced by Candida albicans via pattern recognition receptor interactions are sufficient but not necessary for the acute neutrophil response during experimental vaginal candidiasis.
The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria.
Tumor necrosis factor-α blocks differentiation and enhances suppressive activity of immature myeloid cells during chronic inflammation.
The acute neutrophil response mediated by S100 alarmins during vaginal Candida infections is independent of the Th17-pathway.
MMP-8 deficiency increases TLR/RAGE ligands S100A8 and S100A9 and exacerbates lung inflammation during endotoxemia.
Myeloid-derived suppressor cells infiltrate the heart in acute Trypanosoma cruzi infection.
Dufour A, Bellac CL, Eckhard U, Solis N, Klein T, Kappelhoff R, Fortelny N, Jobin P, Rozmus J, Mark J, Pavlidis P, Dive V, Barbour SJ, Overall CM
Nature communications 2018 Jun 20;9(1):2416
Nature communications 2018 Jun 20;9(1):2416
HIF-1α activation in myeloid cells accelerates dextran sodium sulfate-induced colitis progression in mice.
Kim YE, Lee M, Gu H, Kim J, Jeong S, Yeo S, Lee YJ, Im SH, Sung YC, Kim HJ, Weissman IL, Ahn GO
Disease models & mechanisms 2018 Jul 30;11(7)
Disease models & mechanisms 2018 Jul 30;11(7)
Beneficial Effects of Sodium Phenylbutyrate Administration during Infection with Salmonella enterica Serovar Typhimurium.
Jellbauer S, Perez Lopez A, Behnsen J, Gao N, Nguyen T, Murphy C, Edwards RA, Raffatellu M
Infection and immunity 2016 Sep;84(9):2639-52
Infection and immunity 2016 Sep;84(9):2639-52
Exploring experimental cerebral malaria pathogenesis through the characterisation of host-derived plasma microparticle protein content.
Tiberti N, Latham SL, Bush S, Cohen A, Opoka RO, John CC, Juillard A, Grau GE, Combes V
Scientific reports 2016 Dec 5;6:37871
Scientific reports 2016 Dec 5;6:37871
Loss of JUNB/AP-1 promotes invasive prostate cancer.
Thomsen MK, Bakiri L, Hasenfuss SC, Wu H, Morente M, Wagner EF
Cell death and differentiation 2015 Apr;22(4):574-82
Cell death and differentiation 2015 Apr;22(4):574-82
S100A9 knockout decreases the memory impairment and neuropathology in crossbreed mice of Tg2576 and S100A9 knockout mice model.
Kim HJ, Chang KA, Ha TY, Kim J, Ha S, Shin KY, Moon C, Nacken W, Kim HS, Suh YH
PloS one 2014;9(2):e88924
PloS one 2014;9(2):e88924
Preferential lymphatic growth in bronchus-associated lymphoid tissue in sustained lung inflammation.
Baluk P, Adams A, Phillips K, Feng J, Hong YK, Brown MB, McDonald DM
The American journal of pathology 2014 May;184(5):1577-92
The American journal of pathology 2014 May;184(5):1577-92
The junctional epithelium originates from the odontogenic epithelium of an erupted tooth.
Yajima-Himuro S, Oshima M, Yamamoto G, Ogawa M, Furuya M, Tanaka J, Nishii K, Mishima K, Tachikawa T, Tsuji T, Yamamoto M
Scientific reports 2014 May 2;4:4867
Scientific reports 2014 May 2;4:4867
Neutrophil dependence of vascular remodeling after Mycoplasma infection of mouse airways.
Baluk P, Phillips K, Yao LC, Adams A, Nitschké M, McDonald DM
The American journal of pathology 2014 Jun;184(6):1877-89
The American journal of pathology 2014 Jun;184(6):1877-89
Vaginal epithelial cell-derived S100 alarmins induced by Candida albicans via pattern recognition receptor interactions are sufficient but not necessary for the acute neutrophil response during experimental vaginal candidiasis.
Yano J, Palmer GE, Eberle KE, Peters BM, Vogl T, McKenzie AN, Fidel PL Jr
Infection and immunity 2014 Feb;82(2):783-92
Infection and immunity 2014 Feb;82(2):783-92
The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria.
Behnsen J, Jellbauer S, Wong CP, Edwards RA, George MD, Ouyang W, Raffatellu M
Immunity 2014 Feb 20;40(2):262-73
Immunity 2014 Feb 20;40(2):262-73
Tumor necrosis factor-α blocks differentiation and enhances suppressive activity of immature myeloid cells during chronic inflammation.
Sade-Feldman M, Kanterman J, Ish-Shalom E, Elnekave M, Horwitz E, Baniyash M
Immunity 2013 Mar 21;38(3):541-54
Immunity 2013 Mar 21;38(3):541-54
The acute neutrophil response mediated by S100 alarmins during vaginal Candida infections is independent of the Th17-pathway.
Yano J, Kolls JK, Happel KI, Wormley F, Wozniak KL, Fidel PL Jr
PloS one 2012;7(9):e46311
PloS one 2012;7(9):e46311
MMP-8 deficiency increases TLR/RAGE ligands S100A8 and S100A9 and exacerbates lung inflammation during endotoxemia.
González-López A, Aguirre A, López-Alonso I, Amado L, Astudillo A, Fernández-García MS, Suárez MF, Batalla-Solís E, Colado E, Albaiceta GM
PloS one 2012;7(6):e39940
PloS one 2012;7(6):e39940
Myeloid-derived suppressor cells infiltrate the heart in acute Trypanosoma cruzi infection.
Cuervo H, Guerrero NA, Carbajosa S, Beschin A, De Baetselier P, Gironès N, Fresno M
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 1;187(5):2656-65
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 1;187(5):2656-65
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse S100A8 by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S100A8 at approximately 10-11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse S100A8 by Simple WesternTM. Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A8 at approximately 9 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- S100A8 in NMuMG Mouse Cell Line. S100A8 was detected in immersion fixed NMuMG mouse mammary gland epithelial cell line using 10 µg/mL Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- S100A8 in Mouse Splenocytes. S100A8 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
