NBP2-47667-0.1mg
antibody from Novus Biologicals
Targeting: S100A8
60B8AG, CAGA, CFAG, CGLA, MRP8, P8
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-47667-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal S100A8/A9 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.
- Reactivity
- Human, Mouse, Rat, Canine, Feline, Goat, Guinea Pig, Porcine, Rabbit, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: S100A8/A9 Antibody (MRP8 7C12/4) - Azide and BSA Free [NBP2-47667] - Human Tonsil stained with Macrophage L1 Protein Monoclonal Antibody (MAC387)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: S100A8/A9 Antibody (MRP8 7C12/4) - Azide and BSA Free [NBP2-47667] - Mouse splenocytes were fixed and permeabilized with Foxp3 Fixation/Permeabilization kit. Splenocytes were incubated with Fc block for 5 mins at RT and stained with or without antibody at 1:100 dilution for 25 mins at RT. Data was acquired on a Cytoflex LX and analyzed using FlowJo 10. This image was added from customer review. Image from the Alexa Fluor 488 version of this antibody.