Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- 14-9745-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- S100A8 Monoclonal Antibody (CF-145), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody CF-145 recognizes human S100A8. S100A8, also known as migration inhibitory factor-related protein 8 (MRP-8) or Calgranulin-A, is a calcium binding protein and member of the S100 family of proteins. S100A8 is expressed by neutrophils, monocytes, macrophages, granulocytes, and epithelial cells during dermatoses, such as psoriasis. S100A8 forms a heterodimer with S100A9, which together is known as calprotectin. S100A8 has antimicrobial and pro-apoptotic properties and is a pro-inflammatory mediator in acute and chronic inflammation. Levels of S100A8 are elevated in patients with various inflammatory disorders such as cystic fibrosis, rheumatoid arthritis, and chronic bronchitis. Upregulation of S100A8 is also observed in many tumors, including skin, breast, colon, pancreatic, and ovarian cancers. Applications Reported: This CF-145 antibody has been reported for use in western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This CF-145 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low pH antigen retrieval and can be used at less than or equal to 1 µg/mL. This CF-145 antibody has been tested by western blot of A375 cell lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CF-145
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references High-Plex Spatial RNA Profiling Reveals Cell Type‒Specific Biomarker Expression during Melanoma Development.
S100A8 and S100A9: new insights into their roles in malignancy.
The endogenous Toll-like receptor 4 agonist S100A8/S100A9 (calprotectin) as innate amplifier of infection, autoimmunity, and cancer.
S100A8: emerging functions and regulation.
Kiuru M, Kriner MA, Wong S, Zhu G, Terrell JR, Li Q, Hoang M, Beechem J, McPherson JD
The Journal of investigative dermatology 2022 May;142(5):1401-1412.e20
The Journal of investigative dermatology 2022 May;142(5):1401-1412.e20
S100A8 and S100A9: new insights into their roles in malignancy.
Srikrishna G
Journal of innate immunity 2012;4(1):31-40
Journal of innate immunity 2012;4(1):31-40
The endogenous Toll-like receptor 4 agonist S100A8/S100A9 (calprotectin) as innate amplifier of infection, autoimmunity, and cancer.
Ehrchen JM, Sunderkötter C, Foell D, Vogl T, Roth J
Journal of leukocyte biology 2009 Sep;86(3):557-66
Journal of leukocyte biology 2009 Sep;86(3):557-66
S100A8: emerging functions and regulation.
Passey RJ, Xu K, Hume DA, Geczy CL
Journal of leukocyte biology 1999 Oct;66(4):549-56
Journal of leukocyte biology 1999 Oct;66(4):549-56
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human spleen using 1 µg/mL of Anti-Human S100A8 Purified, followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.