MAB1720

antibody from R&D Systems

Targeting: DSG3 CDHF6

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1720

Provider

R&D Systems

Product name

Human Desmoglein-3 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Desmoglein-3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Desmoglein-1, rhDesmoglein-2, or rhCadherin-17 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P32926


Isotype

IgG

Antibody clone number

216519

Vial size

500 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Oncoproteomic and gene expression analyses identify prognostic biomarkers for second primary malignancy in patients with head and neck squamous cell carcinoma.

Bunbanjerdsuk S, Vorasan N, Saethang T, Pongrujikorn T, Pangpunyakulchai D, Mongkonsiri N, Arsa L, Thokanit N, Pongsapich W, Anekpuritanang T, Ngamphaiboon N, Jinawath A, Sunpaweravong S, Pisitkun T, Suktitipat B, Jinawath N
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2019 Jul;32(7):943-956

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Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection.

Sumitomo T, Mori Y, Nakamura Y, Honda-Ogawa M, Nakagawa S, Yamaguchi M, Matsue H, Terao Y, Nakata M, Kawabata S
Frontiers in cellular and infection microbiology 2018;8:10

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Gene expression profiling of idiopathic interstitial pneumonias (IIPs): identification of potential diagnostic markers and therapeutic targets.

Horimasu Y, Ishikawa N, Taniwaki M, Yamaguchi K, Hamai K, Iwamoto H, Ohshimo S, Hamada H, Hattori N, Okada M, Arihiro K, Ohtsuki Y, Kohno N
BMC medical genetics 2017 Aug 18;18(1):88

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Central role of alpha9 acetylcholine receptor in coordinating keratinocyte adhesion and motility at the initiation of epithelialization.

Chernyavsky AI, Arredondo J, Vetter DE, Grando SA
Experimental cell research 2007 Oct 1;313(16):3542-55

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Desmoglein versus non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling pathways downstream of pemphigus vulgaris antigens.

Chernyavsky AI, Arredondo J, Kitajima Y, Sato-Nagai M, Grando SA
The Journal of biological chemistry 2007 May 4;282(18):13804-12

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Desmoglein-3 by Western Blot. Western blot shows lysates of human skin cancer (squamous cell carcinoma) tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Desmoglein-3 Monoclonal Antibody (Catalog # MAB1720) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Desmoglein-3 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Desmoglein-3 in Human Squamous Cell Carcinoma. Desmoglein-3 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Desmoglein-3 Monoclonal Antibody (Catalog # MAB1720) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.