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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ5307 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- LBR Antibody / Lamin B Receptor
- Antibody type
- Polyclonal
- Description
- This highly specific LBR antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- Store the LBR antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) placenta, 2) HL-60, 3) K562, 4) ThP-1, 5) HEK293, 6) U-2 OS, 7) U937 and 8) Caco-2 lysate with LBR antibody. Predicted molecular weight ~71 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of rat 1) thymus, 2) spleen, 3) testis and mouse 4) thymus, 5) stomach, 6) testis and 7) SP2/0 lysate with LBR antibody. Predicted molecular weight ~71 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human U-2 OS cells with LBR antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer with LBR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U937 cells with LBR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LBR antibody.
