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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-44946 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WNT3A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: MAPLGYFLLL CSLKQALGSY PIWWSLAVGP QYSSLGSQPI LCASIPGLVP Sequence homology: Cow: 79%; Dog: 79%; Guinea Pig: 79%; Horse: 93%; Human: 100%; Mouse: 86%; Pig: 93%; Rabbit: 79%; Rat: 86%; Zebrafish: 79%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Selective Surface and Intraluminal Localization of Wnt Ligands on Small Extracellular Vesicles Released by HT-22 Hippocampal Neurons.
Torres VI, Barrera DP, Varas-Godoy M, Arancibia D, Inestrosa NC
Frontiers in cell and developmental biology 2021;9:735888
Frontiers in cell and developmental biology 2021;9:735888
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- FIGURE 2 Wnt ligand expression levels in HT-22 cells. (A) Comparison of western blot analysis of Wnt3a, Wnt5a, Wnt7a, and GPR177 protein content in cell lysate and P100 fraction. Each lane was loaded with 30 mug protein. (B) Summary densitometric measurement of band intensity showing enrichment (fold) of each protein in the P100 pellet relative to a corresponding lysate. Wnt3a, 0.4 +- 0.19 ( n = 5); Wnt5a, 8.70 +- 4.14 ( n = 7); Wnt7a, 0.22 +- 0.21 ( n = 4; four independent cell culture preparations); GPR177 3.30 +- 1.93 ( n = 3; independent cell culture preparations).
