Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB9025-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Wnt-3a Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse Wnt-3a in direct ELISAs.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P27467
- Isotype
- IgG
- Antibody clone number
- 930769
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references miR-600 Acts as a Bimodal Switch that Regulates Breast Cancer Stem Cell Fate through WNT Signaling.
El Helou R, Pinna G, Cabaud O, Wicinski J, Bhajun R, Guyon L, Rioualen C, Finetti P, Gros A, Mari B, Barbry P, Bertucci F, Bidaut G, Harel-Bellan A, Birnbaum D, Charafe-Jauffret E, Ginestier C
Cell reports 2017 Feb 28;18(9):2256-2268
Cell reports 2017 Feb 28;18(9):2256-2268
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Topflash Reporter Activity Induced by Wnt-3a and Neutralization by Human/Mouse Wnt-3a Antibody. Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induces Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity elicited by Recombinant Mouse Wnt-3a (0.5 μg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse Wnt-3a Monoclonal Antibody (Catalog # MAB9025). The ND50 is typically 0.15-0.9 μg/mL.