Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-19050 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-19050, RRID:AB_1643821
- Product name
- Mouse Monoclonal Wnt-3a Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references WNT/β-catenin Signaling Pathway and Downstream Modulators in Low- and High-grade Glioma.
Denysenko T, Annovazzi L, Cassoni P, Melcarne A, Mellai M, Schiffer D
Cancer genomics & proteomics 2016 Jan-Feb;13(1):31-45
Cancer genomics & proteomics 2016 Jan-Feb;13(1):31-45
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) [NBP1-19050] - Human breast cancer tissue were incubated with anti-human Wn-t3a for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).