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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [3]
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- Product number
- TA502659 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502659, RRID:AB_11124660
- Product name
- GUK1 (Guanylate Kinase 1) mouse monoclonal antibody, clone OTI1D7 (formerly 1D7)
- Antibody type
- Monoclonal
- Description
- GUK1 (Guanylate Kinase 1) mouse monoclonal antibody, clone OTI1D7 (formerly 1D7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GUK1
- Isotype
- IgG
- Antibody clone number
- OTI1D7
- Vial size
- 100 µl
- Concentration
- 0.33 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GUK1 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GUK1 (RC202510, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GUK1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GUK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502659)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-GUK1 antibody(TA502659),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC202510 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GUK1 antibody(TA502659), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-GUK1 antibody(TA502659),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
