Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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Validation data
Reference
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- Product number
- GTX54102 - Provider product page
- Provider
- GeneTex
- Product name
- Histone H3K9me2 (di-methyl Lys9) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer.
Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A.
Zhang K, Wang J, Yang L, Yuan YC, Tong TR, Wu J, Yun X, Bonner M, Pangeni R, Liu Z, Yuchi T, Kim JY, Raz DJ
Molecular cancer 2018 Oct 22;17(1):153
Molecular cancer 2018 Oct 22;17(1):153
Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A.
Eid W, Abdel-Rehim W
Biological chemistry 2016 Nov 1;397(11):1205-1213
Biological chemistry 2016 Nov 1;397(11):1205-1213
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Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- WB analysis of HeLa lysate and H3 protein using Histone H3K9me2 (di-methyl Lys9) antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of 293T cells using Histone H3K9me2 (di-methyl Lys9) antibody (red) and DAPI (blue).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of rat liver tissue using Histone H3K9me2 (di-methyl Lys9) antibody at a dilution of 1:200.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human thyroid cancer tissue using Histone H3K9me2 (di-methyl Lys9) antibody at a dilution of 1:200.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human rectal cancer tissue using Histone H3K9me2 (di-methyl Lys9) antibody at a dilution of 1:200.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of rat lung tissue using Histone H3K9me2 (di-methyl Lys9) antibody at a dilution of 1:200.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of rat testis tissue using Histone H3K9me2 (di-methyl Lys9) antibody at a dilution of 1:200.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of rat brain tissue using Histone H3K9me2 (di-methyl Lys9) antibody at a dilution of 1:200.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of mouse kidney tissue using Histone H3K9me2 (di-methyl Lys9) antibody at a dilution of 1:200.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ChIP assay was performed with 293 lysate and either Histone H3K9me2 (di-methyl Lys9) antibody or rabbit IgG. The precipitated DNA was detected by qPCR with primer set targeting to indicated genes. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Dot-blot analysis of all sorts of methylation peptides using Histone H3K9me2 (di-methyl Lys9) antibody.
- Validation comment
- Dot