ARG54761
antibody from Arigo
Targeting: H3-4
H3.4, H3/g, H3FT, H3t, HIST3H3
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [8]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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Validation data
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- Product number
- ARG54761 - Provider product page
- Provider
- Arigo
- Product name
- anti-Histone H3 dimethyl (Lys4) antibody - ChIP-Grade
- Antibody type
- Polyclonal
- Antigen
- Synthetic methylated peptide around Lys4 of Human histone H3 (NP_003484.1)
- Description
- Affinity purification with immunogen.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Storage
- For continuous use, store undiluted antibody at 4°C for up to weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts of HeLa cell line and H3 protein expressed in E.coli. stained with ARG54761 anti-Histone H3 dimethyl (Lys4) antibody - ChIP-Grade.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: 293T cell stained with ARG54761 anti-Histone H3 dimethyl (Lys4) antibody - ChIP-Grade. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Rat liver stained with ARG54761 anti-Histone H3 dimethyl (Lys4) antibody - ChIP-Grade at 1:100 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded rat lung tissue stained with ARG54761 anti-Histone H3 dimethyl (Lys4) antibody - ChIP-Grade at 1:200 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded human lung cancer tissue stained with ARG54761 anti-Histone H3 dimethyl (Lys4) antibody - ChIP-Grade at 1:200 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded human kidney cancer tissue stained with ARG54761 anti-Histone H3 dimethyl (Lys4) antibody - ChIP-Grade at 1:200 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Mouse brain stained with ARG54761 anti-Histone H3 dimethyl (Lys4) antibody - ChIP-Grade at 1:100 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Mouse lung stained with ARG54761 anti-Histone H3 dimethyl (Lys4) antibody - ChIP-Grade at 1:100 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Rat brain stained with ARG54761 anti-Histone H3 dimethyl (Lys4) antibody - ChIP-Grade at 1:100 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Rat kidney stained with ARG54761 anti-Histone H3 dimethyl (Lys4) antibody - ChIP-Grade at 1:100 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- ChIP: PCR analysis of target genes in 293 cell line lysates after ChIP with ARG54761 anti-Histone H3 dimethyl (Lys4) antibody - ChIP-Grade or control IgG antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Dot-blot: all sorts of methylated peptides stained with ARG54761 anti-Histone H3 dimethyl (Lys4) antibody - ChIP-Grade.