PA5-96111

antibody from Invitrogen Antibodies

Targeting: H3-4 H3.4, H3/g, H3FT, H3t, HIST3H3

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96111

Provider

Invitrogen Antibodies

Product name

Histone H3.1t Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Samples: Jurkat, A-431, Mouse liver, Mouse testis, Rat kidney; Cellular Location: Chromosome, Nucleus Immunogen sequence: VKKPHRYRPG TVALREIRRY QKSTELLIRK LPFQRLVREI AQDFKTDLRF QSSAVMALQE ACEAYLVGLF EDTNLCAIHA KRVTIMPKDI QLARRIRGER A

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.17 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Histone H3.1t was performed in U2OS cells using Histone H3.1t Polyclonal Antibody (Product # PA5-96111).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Histone H3.1t in mouse testis cells. Samples were incubated with Histone H3.1t Polyclonal antibody (Product # PA5-96111) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Histone H3.1t in rat testis cells. Samples were incubated with Histone H3.1t Polyclonal antibody (Product # PA5-96111) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Histone H3.1t in 600 μg extracts of HeLa cells. Samples were precipitated with 10 μg Histone H3.1t Polyclonal antibody (Product # PA5-96111). Western blot was performed from the immunoprecipitate using Histone H3.1t Polyclonal antibody (Product # PA5-96111) at a dilution of 1:30,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Histone H3.1t in 600 μg extracts of HeLa cells. Samples were precipitated with 10 μg Histone H3.1t Polyclonal antibody (Product # PA5-96111). Western blot was performed from the immunoprecipitate using Histone H3.1t Polyclonal antibody (Product # PA5-96111) at a dilution of 1:30,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Histone H3.1t in paraffin-embedded mouse kidney. Samples were incubated with Histone H3.1t Polyclonal antibody (Product # PA5-96111) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Histone H3.1t in paraffin-embedded human brain. Samples were incubated with Histone H3.1t Polyclonal antibody (Product # PA5-96111) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Histone H3.1t in paraffin-embedded rat lung. Samples were incubated with Histone H3.1t Polyclonal antibody (Product # PA5-96111) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Histone H3.1t in paraffin-embedded human liver. Samples were incubated with Histone H3.1t Polyclonal antibody (Product # PA5-96111) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin immunoprecipitation analysis of Histone H3.1t in HeLa cell line using Histone H3.1t Polyclonal Antibody (Product # PA5-96111) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP analysis of Histone H3.1t in extracts of 293T cells. Samples were incubated with Histone H3.1t Polyclonal antibody (Product # PA5-96111) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.