CABT-RM108

antibody from Creative Diagnostics

Targeting: H3-4 H3.4, H3/g, H3FT, H3t, HIST3H3

Western blot (Recommended by provider)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

CABT-RM108

Provider

Creative Diagnostics

Product name

Rabbit Anti-Histone H3Kme3Q5Ser polyclonal antibody

Antibody type

Polyclonal

Description

Histone H3.1 is encoded by the HIST3H3 gene in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. Histone H 3.1 is a core component of nucleosome that is present only in mammals. It is expressed during S phase, then its expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It serves as the canonical histone, which is incorporated during DNA replication. Histone 3.1 can also undergo serotonylation of glutamine at position 5 (Q5ser) in organisms that produce serotonin. This has been demonstrated that tissue transglutaminase 2 can serotonylate histone H3K4me3 marked nucleosomes, which results in the formation of H3K4me3Q5ser. The presence of Q5ser does not affect the ability of MLL1, an H3K4 methyltransferase, to modify nucleosomes in vitro. In the brain the H3K4me3Q5ser signal is not segregated only to regions in which serotonin is produced, but is shown to be broadly distributed. H3K4me3Q5ser expression has also been reported in human pluripotent stem cell-derived serotonergic neurons and with differentiation the levels of H3K4me3Q5ser increase significantly.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 _L, 25 _L

Storage

Stable for 1 year at 2-8°C from date of receipt.