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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-35368 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p73 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Deferasirox-Dependent Iron Chelation Enhances Mitochondrial Dysfunction and Restores p53 Signaling by Stabilization of p53 Family Members in Leukemic Cells.
Rapamycin enhances the sensitivity of ER‑positive breast cancer cells to tamoxifen by upregulating p73 expression.
Calabrese C, Panuzzo C, Stanga S, Andreani G, Ravera S, Maglione A, Pironi L, Petiti J, Shahzad Ali MS, Scaravaglio P, Napoli F, Fava C, De Gobbi M, Frassoni F, Saglio G, Bracco E, Pergolizzi B, Cilloni D
International journal of molecular sciences 2020 Oct 16;21(20)
International journal of molecular sciences 2020 Oct 16;21(20)
Rapamycin enhances the sensitivity of ER‑positive breast cancer cells to tamoxifen by upregulating p73 expression.
Zhu L, Li XX, Shi L, Wu J, Qian JY, Xia TS, Zhou WB, Sun X, Zhou XJ, Wei JF, Ding Q
Oncology reports 2019 Jan;41(1):455-464
Oncology reports 2019 Jan;41(1):455-464
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TP73 in 293 cells (right) compared to a negative control (left) using a TP73 polyclonal antibody (Product # PA5-35368) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of p73 in formalin fixed and paraffin embedded mouse brain. Samples were incubated with p73 polyclonal antibody (Product # PA5-35368) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of TP73 in 293 cells (right) compared to a negative control (left) using a TP73 polyclonal antibody (Product # PA5-35368) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Deferasirox regulates p53 and p73 protein stability. ( A ) Immunofluorescence of p53 and p73 after DFX treatment. The green signal corresponds to p53 or p73 while the red propidium is used to detect nuclei (63x magnification). ( B ) p73 Immunohistochemistry on MDS bone marrow samples derived from 3 different patients at diagnosis (DX) and within one year of iron chelation treatment (ICT) (20x magnification). ( C ) Differentially expressed genes clusters enriched according to Gene Ontology terms and ordered by ascending adjusted p -value. ( D ) Schematic representation of the plausible role of iron chelation on mitochondrial activity and on p53 family stability (created in biorender.com). Abbreviations: NT, not treated; DFX, Deferasirox.
