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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-420 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-420, RRID:AB_10001639
- Product name
- Rabbit Polyclonal p73 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. The epitope is found in isoforms alpha, beta, gamma, delta, epsilon, zeta, and kappa of human p73. The antibody is not expected to react with deltaNp73.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references N-(phosphonacetyl)-L-aspartate induces TAp73-dependent apoptosis by modulating multiple Bcl-2 proteins: potential for cancer therapy.
TAp73 induction by nitric oxide: regulation by checkpoint kinase 1 (CHK1) and protection against apoptosis.
Novel p63 target genes involved in paracrine signaling and keratinocyte differentiation.
Securinine induces p73-dependent apoptosis preferentially in p53-deficient colon cancer cells.
Establishment of a dog model for the p53 family pathway and identification of a novel isoform of p21 cyclin-dependent kinase inhibitor.
A gene signature-based approach identifies mTOR as a regulator of p73.
A regulatory circuit controlling Itch-mediated p73 degradation by Runx.
Aurora kinase A inhibition leads to p73-dependent apoptosis in p53-deficient cancer cells.
Small-molecule RETRA suppresses mutant p53-bearing cancer cells through a p73-dependent salvage pathway.
Differential regulation of vitamin D receptor (VDR) by the p53 Family: p73-dependent induction of VDR upon DNA damage.
DeltaNp73 modulates nerve growth factor-mediated neuronal differentiation through repression of TrkA.
SHP-2 tyrosine phosphatase inhibits p73-dependent apoptosis and expression of a subset of p53 target genes induced by EGCG.
The Yes-associated protein 1 stabilizes p73 by preventing Itch-mediated ubiquitination of p73.
Ruhul Amin AR, Thakur VS, Gupta K, Agarwal MK, Wald DN, Shin DM, Agarwal ML
Oncogene 2013 Feb 14;32(7):920-9
Oncogene 2013 Feb 14;32(7):920-9
TAp73 induction by nitric oxide: regulation by checkpoint kinase 1 (CHK1) and protection against apoptosis.
Tebbi A, Guittet O, Cottet MH, Vesin MF, Lepoivre M
The Journal of biological chemistry 2011 Mar 11;286(10):7873-84
The Journal of biological chemistry 2011 Mar 11;286(10):7873-84
Novel p63 target genes involved in paracrine signaling and keratinocyte differentiation.
Barton CE, Johnson KN, Mays DM, Boehnke K, Shyr Y, Boukamp P, Pietenpol JA
Cell death & disease 2010;1:e74
Cell death & disease 2010;1:e74
Securinine induces p73-dependent apoptosis preferentially in p53-deficient colon cancer cells.
Rana S, Gupta K, Gomez J, Matsuyama S, Chakrabarti A, Agarwal ML, Agarwal A, Agarwal MK, Wald DN
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Jun;24(6):2126-34
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Jun;24(6):2126-34
Establishment of a dog model for the p53 family pathway and identification of a novel isoform of p21 cyclin-dependent kinase inhibitor.
Zhang J, Chen X, Kent MS, Rodriguez CO, Chen X
Molecular cancer research : MCR 2009 Jan;7(1):67-78
Molecular cancer research : MCR 2009 Jan;7(1):67-78
A gene signature-based approach identifies mTOR as a regulator of p73.
Rosenbluth JM, Mays DJ, Pino MF, Tang LJ, Pietenpol JA
Molecular and cellular biology 2008 Oct;28(19):5951-64
Molecular and cellular biology 2008 Oct;28(19):5951-64
A regulatory circuit controlling Itch-mediated p73 degradation by Runx.
Levy D, Reuven N, Shaul Y
The Journal of biological chemistry 2008 Oct 10;283(41):27462-8
The Journal of biological chemistry 2008 Oct 10;283(41):27462-8
Aurora kinase A inhibition leads to p73-dependent apoptosis in p53-deficient cancer cells.
Dar AA, Belkhiri A, Ecsedy J, Zaika A, El-Rifai W
Cancer research 2008 Nov 1;68(21):8998-9004
Cancer research 2008 Nov 1;68(21):8998-9004
Small-molecule RETRA suppresses mutant p53-bearing cancer cells through a p73-dependent salvage pathway.
Kravchenko JE, Ilyinskaya GV, Komarov PG, Agapova LS, Kochetkov DV, Strom E, Frolova EI, Kovriga I, Gudkov AV, Feinstein E, Chumakov PM
Proceedings of the National Academy of Sciences of the United States of America 2008 Apr 29;105(17):6302-7
Proceedings of the National Academy of Sciences of the United States of America 2008 Apr 29;105(17):6302-7
Differential regulation of vitamin D receptor (VDR) by the p53 Family: p73-dependent induction of VDR upon DNA damage.
Kommagani R, Payal V, Kadakia MP
The Journal of biological chemistry 2007 Oct 12;282(41):29847-54
The Journal of biological chemistry 2007 Oct 12;282(41):29847-54
DeltaNp73 modulates nerve growth factor-mediated neuronal differentiation through repression of TrkA.
Zhang J, Chen X
Molecular and cellular biology 2007 May;27(10):3868-80
Molecular and cellular biology 2007 May;27(10):3868-80
SHP-2 tyrosine phosphatase inhibits p73-dependent apoptosis and expression of a subset of p53 target genes induced by EGCG.
Amin AR, Thakur VS, Paul RK, Feng GS, Qu CK, Mukhtar H, Agarwal ML
Proceedings of the National Academy of Sciences of the United States of America 2007 Mar 27;104(13):5419-24
Proceedings of the National Academy of Sciences of the United States of America 2007 Mar 27;104(13):5419-24
The Yes-associated protein 1 stabilizes p73 by preventing Itch-mediated ubiquitination of p73.
Levy D, Adamovich Y, Reuven N, Shaul Y
Cell death and differentiation 2007 Apr;14(4):743-51
Cell death and differentiation 2007 Apr;14(4):743-51
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p73 Antibody [NB100-420] - Detection of Human p73 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-p73 antibody NB100-420 used for WB at 1 ug/ml (A and B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 30 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: p73 Antibody [NB100-420] - FFPE section of human basal cell carcinoma. Affinity purified rabbit anti-p73 used at a dilution of 1:250.
