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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-17161 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Rab4 Monoclonal Antibody (4E11)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17161 targets RAB4A in FACS, IHC, pep-ELISA, and WB applications and shows reactivity with Human, Mouse, and Non-human primate samples.
- Antibody clone number
- 4E11
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAB4A using a RAB4A monoclonal antibody (Product # MA5-17161) against a human RAB4A (AA: 22-218) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of HeLa (Lane 1), MCF-7 (Lane 2), U-87 MG (Lane 3), Jurkat (Lane 4), K562 (Lane 5), A549 (Lane 6), NIH3T3 (Lane 7), PC-12 (Lane 8) and COS-7 (Lane 9). The blot was probed with Anti-RAB4A Monoclonal Antibody (Product # MA5-17161, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 24 kDa band corresponding to RAB4A was observed across all the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAB4A using RAB4A monoclonal antibody (Product # MA5-17161) in Jurkat (1), HeLa (2), A549 (3), HEK293 (4), K562 (5), NIH3T3 (6), PC-12 (7), and COS-7 (8) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of RAB4A was achieved by transfecting HeLa cells with RAB4A specific siRNAs (Silencer® select Product # s11675). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the RAB4A knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with RAB4A Monoclonal Antibody (Product # MA5-17161, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RAB4A.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAB4A using a RAB4A monoclonal antibody (Product # MA5-17161) against a human RAB4A (AA: 22-218) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAB4A using RAB4A monoclonal antibody (Product # MA5-17161) in Jurkat (1), HeLa (2), A549 (3), HEK293 (4), K562 (5), NIH3T3 (6), PC-12 (7), and COS-7 (8) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded lung cancer tissues using RAB4A monoclonal antibody (Product # MA5-17161) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded liver cancer tissues using RAB4A monoclonal antibody (Product # MA5-17161) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using RAB4A monoclonal antibody (Product # MA5-17161) (green) and negative control (red).
