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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
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- Product number
- RQ8319 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NUP133 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific NUP133 antibody is suitable for use in Western blot/Immunofluorescence/Immunoprecipitation/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the NUP133 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) HepG2, 3) 293T and 4) K562 cell lysate with NUP133 antibody. Predicted molecular weight ~129 kDa, commonly observed at ~133 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human K562, 2) human A549, 3) human MCF7, 4) rat C6, 5) rat NRK, 6) mouse Neuro-2a and 7) mouse NIH 3T3 cell lysate with NUP133 antibody. Predicted molecular weight ~129 kDa, commonly observed at ~133 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A549 cells with NUP133 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE rat C6 cells with NUP133 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation of NUP133 protein from 500ug of human HeLa whole cell lysate with 2ug of NUP133 antibody.
