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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PA5-78389 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Orexin B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: mouse brain, rat testis Predicted Reactivity: Human (100%), Rhesus Monkey (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.23 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Orexin B in mouse tissue extract using 50 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with Orexin B polyclonal antibody (Product # PA5-78389) using a dilution of 1:1000 followed by HRP-conjugated anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- hypocretin (orexin) neuropeptide precursor antibody detects hypocretin (orexin) neuropeptide precursor protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with hypocretin (orexin) neuropeptide precursor antibody Orexin B Polyclonal Antibody (Product # PA5-78389) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- hypocretin (orexin) neuropeptide precursor antibody detects hypocretin (orexin) neuropeptide precursor protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with hypocretin (orexin) neuropeptide precursor antibody Orexin B Polyclonal Antibody (Product # PA5-78389) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
