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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- PA5-25142 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERO1LB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans.
Yu XX, Qiu WL, Yang L, Wang YC, He MY, Wang D, Zhang Y, Li LC, Zhang J, Wang Y, Xu CR
Cell research 2021 Aug;31(8):886-903
Cell research 2021 Aug;31(8):886-903
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ERO1LB in MDA-MB435 cell line lysates. Samples were incubated with ERO1LB polyclonal antibody (Product # PA5-25142). Lysates: 35 µg/lane. ERO1LB protein (arrow).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), A549 treated with Thapsigargin (1 µM for 24 hrs) (Lane 2), PC-3 (Lane 3), IM9 (Lane 4), PANC-1 (Lane 5) and AML193 (Lane 6). The blot was probed with Anti-ERO1LB Polyclonal Antibody (Product # PA5-25142, 1:2,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). A 54 kDa band corresponding to ERO1LB was observed in the cell line tested and enhanced upon treatment in A549 cell line.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ERO1LB in formalin fixed and paraffin embedded mouse testis tissue. Samples were incubated with ERO1LB polyclonal antibody (Product # PA5-25142) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ERO1LB in formalin fixed and paraffin embedded mouse testis tissue. Samples were incubated with ERO1LB polyclonal antibody (Product # PA5-25142) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of MDA-MB435 cells using an ERO1LB polyclonal antibody (Product # PA5-25142) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of ERO1LB in MDA-MB435 cells (right histogram). Samples were incubated with ERO1LB polyclonal antibody (Product # PA5-25142) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).
