MA5-34693

antibody from Invitrogen Antibodies

Targeting: LGALS8 PCTA-1

Western blot

Antibody data

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Validation data

Product number: MA5-34693 - Provider product page
Provider: Invitrogen Antibodies
Product name: Galectin 8 Recombinant Rabbit Monoclonal Antibody (JB85-35)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: Positive Control: Mouse testis and rat testis tissue lysates, Human liver tissue, human colon cancer tissue, human kidney tissue, HepG2, A431, PC-3M.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Antibody clone number: JB85-35
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: -20°C, Avoid Freeze/Thaw Cycles, store in dark

LGALS8 protein structure - MA5-34693 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Galectin 8 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), followed by DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Galectin 8 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), followed by DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Galectin 8 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), followed by DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Galectin 8 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), followed by DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Galectin 8 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), followed by DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of paraffin-embedded Galectin 8 in human liver tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS. Incubation was done with monoclonal Galectin 8 antibody (Product # MA5-34693) at a dilution of 1:50 (30 min, RT). The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Galectin 8 in paraffin-embedded human kidney tissue. Samples were incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), and followed by hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Galectin 8 in paraffin-embedded human colon cancer tissue. Samples were incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), and followed by hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of paraffin-embedded Galectin 8 in human kidney tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS. Incubation was done with monoclonal Galectin 8 antibody (Product # MA5-34693) at a dilution of 1:50 (30 min, RT). The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Galectin 8 in paraffin-embedded human liver tissue. Samples were incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), and followed by hematoxylin.