Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-B10785 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ FH / Fumarase / MCL Antibody (aa195-473) LS-B10785
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate). A: HeLa, B: Hep G2 . 10% SDS PAGE. FH / Fumarase antibody diluted at 1:1000
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Fumarate hydratase antibody detects FH protein by Western blot analysis. A. 50 ug mouse liver lysate/extract. 7.5% SDS-PAGE. Fumarate hydratase antibody dilution:1:1000.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Fumarate hydratase antibody detects Fumarate hydratase protein by Western blot analysis. A.50 ug rat liver lysate/extract. 10 % SDS-PAGE. Fumarate hydratase antibody dilution:1:5000
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunofluorescence of paraformaldehyde-fixed HeLa using fumarate hydratase antibody at 1:200 dilution.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunofluorescence of paraformaldehyde-fixed HeLa using fumarate hydratase antibody at 1:200 dilution.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-FH / Fumarase / MCL antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.