H00002271-M09

antibody from Abnova Corporation

Targeting: FH

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002271-M09

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002271-M09, RRID:AB_519154

Product name

FH monoclonal antibody (M09), clone 3E7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant FH.

Antigen sequence

VPSFWPPNAARMASQNSFRIEYDTFGELKVPNDKY
YGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRA
AAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFP
LVVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSK
IPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVL
LPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPL
TLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGT
AVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKF
EALAAHDALVELSGAMNTTACSLMKIANDIRFLGS
GPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTM
VAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVL
HSARLLGDASVSFTENCVVGIQANTERINKLMNES
LMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAI
ELGYLTAEQFDEWVKPKDMLGPK


Isotype

IgG

Antibody clone number

3E7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

FH monoclonal antibody (M09), clone 3E7 Western Blot analysis of FH expression in HeLa ( Cat # L013V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged FH is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to FH on formalin-fixed paraffin-embedded human seminoma. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol