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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-22064 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fumarase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse liver, rat liver.
- Concentration
- 1.08 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of fumarate hydratase using 30 µg of A) HeLa and B) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a fumarate hydratase polyclonal antibody (Product # PA5-22064) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Fumarase was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Fumarase Polyclonal Antibody (Product # PA5-22064) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Fumarase Polyclonal Antibody (Product # PA5-22064). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarase Polyclonal Antibody (Product # PA5-22064) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fumarase Polyclonal Antibody detects FH protein by western blot analysis. A. 50 µg mouse liver lysate/extract. 7.5% SDS-PAGE. Fumarase Polyclonal Antibody (Product # PA5-22064) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fumarase Polyclonal Antibody detects Fumarate hydratase protein by western blot analysis. A.50 µg rat liver lysate/extract.10% SDS-PAGE. Fumarase Polyclonal Antibody (Product # PA5-22064) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of fumarate hydratase showing staining in the mitochondria of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min and stained using a fumarate hydratase polyclonal antibody (Product # PA5-22064) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Fumarase was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: Fumarase Polyclonal Antibody (Product # PA5-22064) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fumarase Polyclonal Antibody detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Fumarate hydratase stained by Fumarase Polyclonal Antibody (Product # PA5-22064) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase (Product # PA5-22064) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
