PA5-18745

antibody from Invitrogen Antibodies

Targeting: FH

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-18745

Provider

Invitrogen Antibodies

Product name

Fumarase Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with canine, mouse and rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 μg

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Fumarase using Fumarase Polyclonal Antibody (Product # PA5-18745) (0.01 µg/mL) in staining of Human Kidney lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA of Fumarase using two Fumarase antibodies. The reporter antibody (Product # PA5-18745) was used at a concentration of 1.5 µg/ml and the reporter antibody was used at a concentration of 2 µg/ml.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Fumarase in human heart. Samples were incubated with Fumarase polyclonal antibody (Product # PA5-18745) using a dilution of 3.75 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Fumarase in human skeletal muscle. Samples were incubated with Fumarase polyclonal antibody (Product # PA5-18745) using a dilution of 3.75 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Fumarase in human skeletal muscle. Samples were incubated with Fumarase polyclonal antibody (Product # PA5-18745) using a dilution of 3.75 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.