Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP1804a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP1804a, RRID:AB_636750
- Product name
- LC3 Antibody (APG8C) (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Triterpenes from Ganoderma Lucidum induce autophagy in colon cancer through the inhibition of p38 mitogen-activated kinase (p38 MAPK).
A novel mTOR activating protein protects dopamine neurons against oxidative stress by repressing autophagy related cell death.
Allopregnanolone treatment delays cholesterol accumulation and reduces autophagic/lysosomal dysfunction and inflammation in Npc1-/- mouse brain.
Thyagarajan A, Jedinak A, Nguyen H, Terry C, Baldridge LA, Jiang J, Sliva D
Nutrition and cancer 2010;62(5):630-40
Nutrition and cancer 2010;62(5):630-40
A novel mTOR activating protein protects dopamine neurons against oxidative stress by repressing autophagy related cell death.
Choi KC, Kim SH, Ha JY, Kim ST, Son JH
Journal of neurochemistry 2010 Jan;112(2):366-76
Journal of neurochemistry 2010 Jan;112(2):366-76
Allopregnanolone treatment delays cholesterol accumulation and reduces autophagic/lysosomal dysfunction and inflammation in Npc1-/- mouse brain.
Liao G, Cheung S, Galeano J, Ji AX, Qin Q, Bi X
Brain research 2009 May 13;1270:140-51
Brain research 2009 May 13;1270:140-51
No comments: Submit comment
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "The anti-LC3 (APG8c) Pab (Cat. #AP1804a) is used in Western blot to detect LC3 (APG8c) in, from left to right, 293, Ramos, Hela, HepG2, T47d, and Jurkat tissue lysates. LC3 (APG8c)(arrow) was detected using the purified Pab."
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
