LS-C287067

antibody from LSBio

Targeting: NF1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C287067

Provider

LSBio

Product name

Anti-Neurofibromin / NF1 Antibody (C-Terminus) LS-C287067

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Rat, Bovine, Canine, Horse, Rabbit

Host

Rabbit

Isotype

IgG

Vial size

100µl

Storage

Store at 2-8°C for up to 1 year.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human and Mouse NF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human NF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NF1 antibody used for IP at 6 ug/mg lysate. NF1 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated NF1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.