A00043-1

antibody from Boster Biological Technology

Targeting: NF1

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00043-1

Provider

Boster Biological Technology

Product name

Anti-Neurofibromin/NF1 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for Neurofibromin/NF1 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

mTORC1 signaling is essential for neurofibromatosis type I gene modulated osteogenic differentiation of BMSCs.

Li Y, Li J, Zhou Q, Liu Y, Chen W, Xu H
Journal of cellular biochemistry 2019 Mar;120(3):2886-2896

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of A549 cells using anti-NF1 antibody (A00043-1). Overlay histogram showing A549 cells stained with A00043-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NF1 Antibody (A00043-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image