Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA1-084 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NF1 Monoclonal Antibody (McNFn27a)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA1-084 detects neurofibromin in human, rat and mouse cells. MA1-084 has been successfully used in Western blot, procedures. By Western blot, this antibody detects a ~230-260 kDa protein. The MA1-084 immunogen is a synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- McNFn27a
- Vial size
- 200 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of neurofibromin from rat brain at ~230 kDa using Product # MA1-084.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Neurofibromin was performed using 70% confluent log phase Neuro-2A cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NF1 (McNFn27a) Mouse Monoclonal Antibody (Product # MA1-084) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.