PA1-46246

antibody from Invitrogen Antibodies

Targeting: NF1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-46246

Provider

Invitrogen Antibodies

Product name

NF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

In Western blot, a band-set at 220-240 kDa and a higher MW band at 450 kDa (uncharacterized) can be seen. For IHC, antigen retrieval is not required for immunostaining of frozen sections. Antigen retrieval with trypsin or citrate improves immunostaining of paraffin sections.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

500 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NF1 in Nf1 -/- knockout fibroblast, normal rat Schwann, normal human Schwann cell extracts. Sample was incubated in NF1 polyclonal antibody (Product # PA1-46246).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of NF1 in maturing, hypertrophic cartilages, and proliferative cartilage. Samples were incubated in NF1 polyclonal antibody (Product # PA1-46246). NF1 protein labeling displays positive signal in the maturing and the hypertrophic cartilages but only faint labeling in the proliferative cartilage.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of NF1 in maturing, hypertrophic cartilages, and proliferative cartilage. Samples were incubated in NF1 polyclonal antibody (Product # PA1-46246). NF1 protein labeling displays positive signal in the maturing and the hypertrophic cartilages but only faint labeling in the proliferative cartilage.