604001

antibody from BioLegend

Targeting: DNM1 DNM

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

604001

Provider

BioLegend

Proper citation

BioLegend Cat#604001, RRID:AB_2716194

Product name

Purified anti-Dynamin-1

Antibody type

Monoclonal

Reactivity

Mouse

Antibody clone number

25 µg

Vial size

0.5 mg/ml

Concentration

2°C-8°C

Storage

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total lysates (15 µg protein) from Raji (negative control), A549 and mouse brain were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-Dynamin-1 antibody (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP goat anti-mouse-IgG secondary antibody for anti-Dynamin-1 antibody or HRP Donkey anti-rabbit IgG Antibody for GAPDH antibody. 1:2000 dilution of GAPDH (poly6314) antibody was used as a loading control (lower). Lane M: MW ladder.

Conjugate

Unconjugated