MA5-34957

antibody from Invitrogen Antibodies

Targeting: DNM1 DNM

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34957

Provider

Invitrogen Antibodies

Product name

Dynamin 1 Monoclonal Antibody (A1A12)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: PC-3M cell lysates, MG-63, SiHa, A549.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

A1A12

Vial size

100 µL

Concentration

2 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles, store in dark

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Dynamin 1 in SiHa cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Dynamin 1 monoclonal antibody (Product # MA5-34957) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Dynamin 1 in SiHa cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Dynamin 1 monoclonal antibody (Product # MA5-34957) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Dynamin 1 in MG-63 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Dynamin 1 monoclonal antibody (Product # MA5-34957) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Dynamin 1 in MG-63 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Dynamin 1 monoclonal antibody (Product # MA5-34957) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Dynamin 1 in SiHa cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Dynamin 1 monoclonal antibody (Product # MA5-34957) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:1000.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Dynamin 1 in A549 cells, unlabelled sample (control; cells without incubation with primary antibody; black). Samples were incubated with Dynamin 1 monoclonal antibody (Product # MA5-34957) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated Goat anti-Mouse IgG with a dilution of 1:1000 (30 min).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Dynamin 1 in A549 cells, unlabelled sample (control; cells without incubation with primary antibody; black). Samples were incubated with Dynamin 1 monoclonal antibody (Product # MA5-34957) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated Goat anti-Mouse IgG with a dilution of 1:1000 (30 min).