NB300-210

antibody from Novus Biologicals

Targeting: DNM1 DNM

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-210

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-210, RRID:AB_2093506

Product name

Sheep Polyclonal Dynamin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specific for the ~95k dynamin protein phosphorylated at Ser778. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by lambda-phosphatase treatment.

Reactivity

Mouse, Rat

Host

Sheep

Isotype

IgG

Vial size

0.1 ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

RCAN1 links impaired neurotrophin trafficking to aberrant development of the sympathetic nervous system in Down syndrome.

Patel A, Yamashita N, Ascaño M, Bodmer D, Boehm E, Bodkin-Clarke C, Ryu YK, Kuruvilla R
Nature communications 2015 Dec 14;6:10119

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Cdk5 is essential for synaptic vesicle endocytosis.

Tan TC, Valova VA, Malladi CS, Graham ME, Berven LA, Jupp OJ, Hansra G, McClure SJ, Sarcevic B, Boadle RA, Larsen MR, Cousin MA, Robinson PJ
Nature cell biology 2003 Aug;5(8):701-10

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Dynamin [p Ser778] Antibody [NB300-210] - Analysis of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 dynamin. The immunolabeling is completely eliminated by treatment.