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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- GTX121956 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX121956, RRID:AB_11168959
- Product name
- SMYD3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE GTX121956 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SMYD3 antibody immunoprecipitates SMYD3 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 2.5 £gg of pre-immune rabbit IgG B : Immunoprecipitation of SMYD3 by 2.5 £gg of SMYD3 antibody (GTX121956) 10% SDS-PAGE The immunoprecipitated SMYD3 protein was detected by SMYD3 antibody (GTX121956) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SMYD3(GTX121956) antibody at 1:250 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HepG2 chromatin extract and 5 £gg of either control rabbit IgG or anti-SMYD3 antibody. The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8.
