AP7344c

antibody from Abcepta

Targeting: SMYD3 KMT3E, ZMYND1, ZNFN3A1

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7344c

Provider

Abcepta

Proper citation

Abgent Cat#AP7344c, RRID:AB_1968309

Product name

SMYD3 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of SMYD3 antibody (Center) (Cat.#AP7344c) in CEM cell line lysates (35ug/lane). SMYD3 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Western blot analysis of lysate from HT-29 cell line, using SMYD3 Antibody (Center)(Cat. #AP7344c). AP7344c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug."

Primary Ab dilution

1:1000

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

SMYD3 Antibody (Center) (Cat. #AP7344c) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50